Vitamin D deficiency self-test

With the Vitamin D Rapid Test Cassette, you can quickly check your vitamin D levels with just a small drop of blood from your fingertip. Low levels may indicate a deficiency, which can affect your health.

This test gives you an initial indication of whether your vitamin D levels may be too low. It is not a complete diagnosis, and only a doctor can confirm your results and advise you on the appropriate treatment if necessary.

Always discuss your results with a doctor or other healthcare professional. The Vitamin D Rapid Test Cassette is a useful tool for health monitoring at home, but it does not replace professional medical advice or a consultation with your doctor.

What is vitamin D deficiency?

Vitamin D deficiency occurs when the body does not get enough of this essential nutrient. Vitamin D is essential for maintaining healthy bones, supporting the immune system, and promoting overall well-being.

Approximately half of all adults in Europe have low vitamin D levels. This is because our bodies mainly produce vitamin D through the skin when it comes into contact with sunlight. During the winter months, or when you spend a lot of time indoors, your body may not produce enough vitamin D.

HOW CAN A DEFICIENCY BE PREVENTED?

The most effective way to prevent vitamin D deficiency is a combination of a balanced diet and safe exposure to the sun. Consult a doctor to find out how much sunlight your skin type can tolerate. It may also be helpful to include foods rich in vitamin D in your diet and talk to a health professional about taking supplements.

Symptoms of vitamin D deficiency

The symptoms of vitamin D deficiency can be vague and develop gradually. Many people do not have any obvious symptoms. Common symptoms include:

  • Fatigue and lack of energy, especially in winter
  • Bone pain or muscle weakness
  • More frequent infections
  • Mood swings, such as seasonal depression or gloominess

Causes of vitamin D deficiency

Various factors can lead to a shortage.

  • Limited exposure to sunlight: The main cause is insufficient exposure to sunlight. This often occurs in winter, when the sun is less strong and we spend less time outdoors.
  • Age: Older people produce less vitamin D through their skin. Their kidneys also absorb vitamin D less effectively.
  • Nutrition: A diet low in vitamin D-rich foods can contribute to lower vitamin D levels.
  • Health status: Certain medical conditions, such as Crohn's disease or celiac disease, can affect the body's ability to absorb vitamin D.

Consequences of a prolonged shortage

A prolonged vitamin D deficiency can have serious health consequences. In children, it can lead to rickets, a condition that causes soft, weak bones. In adults, it can contribute to osteomalacia, which causes bone weakening, and osteoporosis, a condition characterized by brittle bones.

PROCEDURE

  1. Wash your hands with soap and rinse them with clear warm water.

  2. Allow the pouch to reach room temperature before opening it. Open the pouch, remove the test cassette, and place it on a clean, flat surface. Perform the test within one hour. The best results are obtained when the test is performed immediately after opening the foil pouch.
    Remove the dropper, buffer vial, lancet, and alcohol wipe and place them close to the test cassette.

  3. Carefully pull off the cap from the lancet and discard it.

  4. Use the alcohol wipe provided to clean the tip of your middle or ring finger as the puncture site. Allow it to air dry.

  5. Press the lancet in on the side where the cap came off; the tip retracts automatically and safely after use. Massage the hand without touching the puncture site by massaging the hand in the direction of the fingertip of the middle or ring finger to be pricked.

  6. Keep your hand down and massage the pricked area to obtain a drop of blood.

  7. Touch the bulb of the capillary dropper to the blood without squeezing it. The blood will flow through the capillary action in the capillary dropper up to the line marked on the capillary dropper.
    You can massage your finger again to obtain more blood if the blood does not reach the marked line. Avoid air bubbles.

  8. Squeeze the dropper bulb to allow the collected blood to flow into the specimen opening (S) of the cassette.

  9. Wait until the blood has completely entered the well. Unscrew the cap from the buffer bottle and add 2 drops of buffer to the buffer gut (B) of the cassette and start a timer.

  10. Wait until the colored line(s) appear(s). Read the results after 10 minutes. Compare the intensity of the T line with the "Vitamin D color chart" provided with the kit to determine the Vitamin D level in your blood. Do not interpret the result after 20 minutes.

READING THE RESULTS

25-OH Vitamin D level

Reference range (ng/mL)

Reference range  (nmol/L)

Refer to the vitamin D color chart to read the results.

Inadequate

0-10

0-25

Two colored lines will appear. Onewill be in the control area (C) and the other in the test area (T).

If the intensity of the line in the test area (T)is equal to the Deficiency line (0-10 ng/ml) on the color chart, this indicates a vitamin D deficiency.

Insufficient

10:30

25-75

Two colored lines will appear. One will be in the control area (C) and the other should be in the test area (T).

   

If the intensity of the line in the test area (T) is equal to the Insufficient line (10-30 ng/mL) onthe color chart, this means that the vitamin D level is insufficient.

Sufficient

30-100

75-250

Two colored lines will appear. One line must always be in the control area (C), and a faint colored line will appear in the test area (T). If the intensity of the line in the test area (T) is equal to the Sufficient line(30-100 ng/mL) on the color chart, this means that the vitamin D level is sufficient.

Excess

One colored line appears in the control line area (C). No colored line appears in the test line area (T). This means that the vitamin D level may be too high and that it is advisable to consult a doctor.

Invalid

The control line does not appear. Incorrect procedure techniques are the most likely reasons for the control line failure. Review the procedure and repeat the test with a new test. If the problem persists, stop using the test kit immediately and contact your local distributor. 

Frequently Asked Questions

Do you have a question about one of our self-tests? Below you’ll find answers to the most frequently asked questions.
If your question isn’t listed here, please feel free to contact us at info@quantivadx.eu.

In medicine, 25-hydroxyvitamin D is the most important storage form of vitamin D in the body. Therefore, the overall vitamin D status can be determined by measuring the level of 25-hydroxyvitamin D. A 25-hydroxyvitamin D level of less than 30 ng/ml in a positive result indicates a vitamin D deficiency. In these cases, vitamin D supplements may be recommended.

The clinical application of 25-hydroxyvitamin D is primarily intended for the diagnosis, treatment, and monitoring of rickets (in children), osteomalacia, postmenopausal osteoporosis, and renal osteopathy. Vitamin D deficiency is also associated with numerous other conditions, including cancer, cardiovascular disease, autoimmune diseases, diabetes, and depression. Check your vitamin D levels to determine whether you need to take vitamin D supplements. The vitamin D rapid test can be used at any time of day.

The results are accurate, provided that the instructions are followed carefully. However, the result may be incorrect if the Vitamin D Rapid Test cassette gets wet before the test is performed, if there is insufficient blood in the sample tray, or if fewer than 2 or more than 3 drops of buffer are applied. The capillary dropper included in the package allows you to check whether the blood volume collected is correct. In addition, due to the immunological principles involved, there is a rare chance of false results. For such tests based on immunological principles, a consultation with a doctor is always recommended.

Refer to the image and compare the intensity of the T line with the "Vitamin D color chart" included in the kit.

No. The result must be read 10 minutes after adding the buffer. After 20 minutes, the result is unreliable.

If the result is inadequate or insufficient, this means that the vitamin D level in your blood is lower than 30 ng/ml and that you should consult a doctor to show them the test result. The doctor will decide whether additional tests need to be done.

If the result is satisfactory, this means that the vitamin D level is higher than or equal to 30 ng/ml and within the normal range. Although cases of vitamin D poisoning (hypercalcemia) are rare, they cannot be ruled out based on such test results. However, if symptoms persist, it is recommended to consult a doctor.